One of the most time-consuming and expensive components of compiling components in the lab is the massive project of creating the appropriate ADP kinase assays required. A mix and read option can simplify the process and remove a lot of uncertainly and stress from the process. At Bellbrook Labs, you will find transcreener kinase assays which provide exactly that while being compatible with many of the common plate readers that may be found in your facility.
Kinase Assay Kits Applications
Having the right transcreener kinase assay on hand will remove a lot of time from the process of researching to develop members of a family of enzymes which can then detect the ADP, which is the invariant product. The highest quality transcreener kinase assay will offer you the following:
* The ability to determine both inhibitor potency and IC50 values
* The ability to screen for tiny molecular inhibitors
* Mechanisms that allow for action studies
* An option for determining any amount of enzymatic activity
* A way to measure resident off-rate/time dissociation of the target’s lead molecules
The Use of Kinase Assay Kits
When using a kinase assay, what is needed is a straightforward option for detecting the presence of ADP. A specific antibody provides this ability and can bring with specificity and affinity to ADP while having a lesser reactivity to ATP. This is the best way to ensure detection of ADP when there is a much larger amount of ATP. Having the ADP antibody available makes it possible for detection and sensitivity of ADP down to a measure of 10 nM while there is a Z value of at least 0.7.
The best ADP kinase assays are going to be available in homogenous mix and read formats that allow a high degree of automation without dropping the level of flexibility available. It’s also important to consider the detection modes available and whether they fit your needs. The kinase assays from Bellbrook Labs are available with three detection modes and can be run on many different readers. This ensures the best outcome no matter what hardware you use.
If you are looking for ADP kinase assays for your lab, Bellbrook Labs is here to help. If you are interested in learning more about our products or wish to ask a question, you can visit our website at www.BellbrookLabs.com.
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