The Anti-CD3 antibody can react with intracytoplasmic portions of the antigen by the same name, expressed by the T cells. It can stain human T cells in the medulla and cortex of the thymus, as well as in peripheral lymphoid tissue. It can stain normal and neoplastic T cells, as well.
More Information
The Anti-CD3 antibody can be found in polyclonal and monoclonal varieties. The polyclonal version has no clone, and the immunogen is the synthetic 13-mer peptide that corresponds to the human CD3 protein in the epsilon chain. The isotype is the Rabbit IgG, and the molecular weight is 19kDa.
The monoclonal versions have a clone of SP162 or SP7. Both of these have the same immunogen as the polyclonal version, as well as the same isotype. However, the molecular weight of the monoclonal varieties is 20kDa. All have an undetermined epitope.
Applications
The polyclonal antibody can use Immunohistochemistry and Western Blotting applications. The monoclonal versions can also use IHC and Western Blotting, but they can also use Flow Cytometry.
For IHC applications, you’ll want to use Formalin-fixed or paraffin-embedded tissues and deparaffinized slides. You can find a concentrated format of the antibody, as well as pre-diluted versions. Concentrated versions will require dilution using a ratio of 1:150 for monoclonal versions. The dilution rate for the polyclonal is 1:200.
To retrieve the antigen, you’ll need to boil the section of tissue in a citrate buffer with a pH of 6.0 for at least ten minutes and then allow it to cool for 20 minutes. Incubation periods are 10 minutes while at room temperature. The positive control is the tonsil.
The Anti-CD3 antibody can be used for a variety of testing purposes and can be highly valuable to your work. Visit Spring Bioscience now for more information.
