The FOXP3 antibody is necessary for normal immune homeostasis and is considered a 431 amino acid protein. It’s a member of the winged-helix/forkhead family of transcriptional regulators, and it is conserved among all mammals. It not only is expressed at higher levels in CD25_CD4 positive T-cells but also at low concentrations in CD4 positive and CD25 negative cells. However, it is absent in CD4 negative and CD8 positive T-cells. It is considered to be a master regulatory gene and a particular marker for regulatory T-cells.
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It comes in a polyclonal and monoclonal antibody type. The monoclonal antibody has a clone of SP97, and everything else is the same for all styles of FOXP3 antibodies. It is designed to be used for research, and the immunogen is the synthetic peptide that corresponds to the C-terminus for human FOXP3 proteins. The IG isotype is the Rabbit IgG, and its epitope is undetermined. It has a molecular weight of 50kDa and has been human tested.
Its primary application is for Immunohistochemistry. The procedure for IHC is to use formalin-fixed or paraffin-embedded tissues and ensure that slides are deparaffinized appropriately. When using the concentrated formula, you should dilute it using a ratio of 1:100.
For antigen retrieval, you should boil tissues sections in EDTA with a pH of 8.0 for 10 minutes, allowing it to cool to room temperature for 20 minutes. Likewise, incubation periods are 30 minutes at room temperature.
The positive control is the tonsil with cellular localization occurring in the nucleus.
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You can find the FOXP3 antibody in three forms, including 0.1 ml, 0.5 ml, and 1.0 ml. These antibodies will be in a PBS/1 percent BSA buffer with a pH of 7.6 and less than 0.1 percent sodium azide. It also comes in a pre-diluted format of 7.0 ml. Visit Spring Bioscience at for more details.
